Author: Roberta Closter
Unit: 10th Grade Genetics

Title: RNA and Protein Inquiry Lab


Lesson Overview:

Students will have to create their own lab based on the previous lab that we did. They will have to use their knowledge of RNA transcription and the process of translation to make a protein chain. Using the models from the previous lab, I want them to create an RNA strand, edit the RNA, Make tRNA and translate the protein. There will be questions after the lab that I want them to complete and hand in the next day.

Objectives:

Students will be able to create RNA by manipulating a model
Students will be able to edit their RNA strand (model)
Students will be able to make tRNA by manipulating a model
Students will be able to translate proteins of a model

Materials:

DNA models from previous lab
Lab Questions

Links to Resources:

il

Safety Issues:

None

Instruction:


Opening: I will begin lab by telling the students that they are doing an inquiry based lab and that they can come up with their own procedure. I will tell them not to panic and will refer to the board where I will have some guidance written for them. From the previous labs DNA that we made, I want them to construct and manipulate an RNA strand, edit that RNA, Make tRNA and translate the protein. From their prior knowledge, they will have to make their own procedure and answer questions once they have completed the lab. To activate their prior knowledge, from the previous lab I will ask students if they remember building the DNA model out of clay and toothpicks? I will ask what they clay represented and what the toothpicks represented? Then I will ask students what the function of DNA is? Then I will ask if they remember the base pairing rules and have them tell me what the rules are for both DNA and RNA and ask how they are different. Then I will ask what the function of RNA is and how it works? The goal of this inquiry lab is to manipluate RNA, edit it meaning change the base pairs to create /translate different proteins to see how the process of transctiption and translation work within the cell. Doing it ourselves helps to see and do it instead of just reading it in a book, we actually get to manipulate the DNA model we used previously to get these points across. The students will be able to come up with their own procedure based on the given goals to complete and then answer the questions based on their manipulations.

Learning Activities:
  • Students will conduct their own lab today by making their own procedure based on the goals i set forth, their lab questions, and their prior knowledge, books and notes.
  • Before they begin, I will have a pre-constructed model to show them of what they are expected to accomplish.
From the DNA model that we made in our previous lab and knowledge of RNA transcription and process of translation I want them to make a protein chain, without telling them how to do it but for them to complete the lab based on set objectives or goals specifically:
    • create RNA by manipulating a model
    • edit their RNA strand (model)
    • make tRNA by manipulating a model
    • translate proteins of a model / make protein chain
  • They will use the same materials as they did in the previous lab.
  • They can use their books and their notes but they have to come up with their own procedure to fulfill this task and then answer the questions (which can be done in class or finished for homework) based on what I want them to accomplish.
  • The goals of the class are to construct/manipulate an RNA strand, edit the strand, make tRNA and translate the protein. I will be circling the classroom and talking with each group to make sure they are on task and guide anyone that needs help.

Closing: I want to close by having a quick group discussion. I want to see how the students felt by creating their own procedure. "Based on the given information about what I wanted you to accomplish and seeing the model, were you comfortable coming up with your own procedure for this lab"? Then I will ask each group what kind of proteins they made. This leads into: each group teaching the class about how they made their protein chain and show eachther their models. I will tell them if they didn't finish their questions to do so as homework and that they should be sure to look over their notes from the unit to prepare for their essay exam coming up and using the questions from this lab which could possibly be used on their essay exam.

Assessment:

Lab Questions

Homework:


Study!

Additional Notes